ABSTRACT
The main pathogenesis of functional constipation in children is the disturbance of large intestine conduction function, so the treatment of lubricating bowel and purging stool, dispersing food and abducting stagnation, nourishing blood and enriching yin is used in clinic. According to the characteristics of the physiopathologic of children, based on the clinical practice, this article believed that the occurrence of this disease was closely related to the ascending the clear of the spleen, the descending turbidity of the stomach, the dispersion of the lung and the catharsis of the liver. The root of pathogenesis is disturbance in ascending and descending of the functional activities of qi, so it is effective to treat the disease with the theory of qi ascending and descending.
ABSTRACT
Objective To stimulate the expansion of natural killer cells (NK) in vivo, to achieve high expression of Flt-3 ligand ( FL) in the mouse liver by hydrodynamic injection technology , to obtain high purity of NK for cell therapy and then to purify the spleen lymphocytes by MicroBeads sorting ( MACS ) technique.Methods C57BL/6 mice were repeatedly treated with hydrodynamic injection of FL expression plasmid .Then, surface markers of splenic NK were analyzed by flow cytometry and purified by MACS technique .Results Compared with mice treated with one hydrodynamic injection, the spleen of mice treated with two hydrodynamic injections showed significant variation, with the absolute num-ber of lymphocytes increased (6.02 ±1.15) times, the proportion of NK subsets increased (2.07 ±0.39) times, and the absolute number of NK subsets increased (12.49 ±2.39) times.Cell surface marker detection confirmed that NK activity and surface markers did not change significantly .NK with a purity of about (83.81 ±0.92)％ was obtained by the combination of two magnetic beads sorted with CD 5 ( Ly-1) MicroBeads Positive selection and EasySep TM Mouse NK Cell Isolation Kit negative selection .Conclusion NK can be expanded in the spleen of mice by hydrodynamic injection of FL plasmid without influence on the NK activity and surface markers.The combined use of CD5 (Ly-1) MicroBeads positive selection with EasySep TM Mouse NK Cell Isolation Kit negative selection can effectively remove T , B and other miscellaneous cells, thereby contributing to obtaining high-purity NK. This study provides good reference for the immunotherapy of tumor cells.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of thrombocytopenia on the migration patterns of adoptive dendritic cell(DC) in vivo.</p><p><b>METHODS</b>The mouse model of thrombocytopenia was established by intraperitoneal administration of anti-CD41 mAb MWReg30. Mouse bone marrow(BM)-derived DC were injected into thrombocytopenia mouse by footpad infusion and intravenous infusion. The DC migration and distribution pattern were detected by bioluminescence imaging.</p><p><b>RESULTS</b>More than 80% platelets were cleared in the experimental group which was infused with anti-CD41 antibody. At 72 h after injection, the percentage of injected DC that migrated from footpad to popliteal lymph nodes(PLNs) and inguinal lymph nodes(ILNs) were (0.32±0.02)% and (0.02±0.01)% in experimental group, and (0.27±0.15)% and (0.02±0.02)% in control group, respectively. Statistic data showed that there was no statistical difference between these 2 groups (P>0.05). The issue distribution pattern of intravenously injected DC between experimental group and control group were not distinctly different, and large amounts of injected DC accumulated in the spleen, liver draining lymph-nodes lungs and liver.</p><p><b>CONCLUSION</b>Thrombocytopenia has not a distinct effect on the migratory capacity and tissue distribution of DC by either footpad or intravenous injection.</p>
ABSTRACT
<p><b>OBJECTIVE</b>To understand the blood lead level and its influencing factors of workers in one lead acid storage cell enterprise in Jiangsu Province.</p><p><b>METHODS</b>An occupational health field investigation was done to this storage cell enterprise at the end of June 2011 to measure the air lead fume (dust) concentration of workplaces. Health-care information of 1364 person-times from 2009 - 2011 was collected, including blood lead level, general state of health, life and health habit. One way ANOVA and ordinal multi-categorical logistic stepwise regression were used to analysis the influencing factors of blood lead level.</p><p><b>RESULTS</b>The lead fume concentration range was 0.008-0.354 mg/m(3) among 12 measuring points, which 7 places were unqualified, while the concentration range of lead dust was 0.023 - 2.432 mg/m(3), 24 out of 27 measuring places were unqualified, both the qualified rate were low. The blood lead concentration of objects was (259.54 ± 106.62) µg/L, among which 96 people (7.04%) who ≥ 400 µg/L should be identified as suspected "observation object", blood lead concentration ≥ 600 µg/L was not found. The blood lead concentration of male (279.76 ± 114.93 µg/L) was significantly higher than female (242.44 ± 95.86) µg/L (t = 6.441, P < 0.01). The proportion of ≥ 400 µg/L in male (11.04%, 69/625) was significantly higher than female (3.65%, 27/739) (χ(2) = 28.237, P < 0.01). The blood lead concentration of workers who exposed to lead fume or dust (265.93 ± 103.70) µg/L was significantly higher than those of not exposed to lead (205.30 ± 115.62) µg/L (t = -6.037, P < 0.01), the blood lead concentration of workers who exposed to lead dust was (267.38 ± 98.02) µg/L significantly higher than those of exposed to lead fume (260.81 ± 121.80) µg/L (t = -2.408, P < 0.05). The proportion of ≥ 400 µg/L in workers who exposed to lead fume (dust) (7.60%, 93/1223) was significantly higher than those of not exposed to lead (2.13%, 3/141) (χ(2) = 4.538, P < 0.05). Ordinal multi-categorical logistic stepwise regression found that the lead fume concentration ≥ 0.03 mg/m(3), lead dust concentration ≥ 0.05 mg/m(3) (OR = 1.59, 95%CI: 1.06 - 2.39), length of service ≥ 3 years (OR = 1.82, 95%CI: 1.12 - 2.98), smoking (OR = 2.06, 95%CI: 1.27 - 3.37) can increase the level of blood lead concentration.</p><p><b>CONCLUSIONS</b>Lead dust concentration of the enterprise exceeded the standard. Workers exposed to lead fume (dust) have more occupational health hazard of lead, of whom the blood lead concentration was high. Higher lead fume (dust) concentration in workplace, longer length of service, smoking were risk factors of high blood lead concentration.</p>